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stop solution from the silver sequencetm dna sequencing kit  (Promega)

 
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    Promega stop solution from the silver sequencetm dna sequencing kit
    Stop Solution From The Silver Sequencetm Dna Sequencing Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/silver+sequencetm+dna+sequencing+kit/silver+sequence+dna+sequencing+system/pmc06568318-263-10-19
    Average 90 stars, based on 1 article reviews
    stop solution from the silver sequencetm dna sequencing kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted repeats in Streptomyces plasmid pSV1.
    Article Snippet: .. The sequence ladder was made using the Silver SequenceTM DNA Sequencing kit (Promega) with the labelled primer P843. .. Overexpression and purification of RepA protein The pSV1 repA gene (428–913 bp) was cloned into the NdeI and EcoRI sites of E. coli plasmid pET28b (Novagen) to generate pCY40, and then, it was introduced into E. coli strain BL21 (DE3).

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes
    Article Snippet: .. The sequencing ladder is generated from the PCR DNA template by employing the same end-labelled primer used for reverse transcription ( section 6.1.1.2 ) using the Silver sequenceTM DNA sequencing kit (Promega) according to the manufacturer instructions. ..

    Article Title: Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted repeats in Streptomyces plasmid pSV1.
    Article Snippet: .. The sequence ladder was made using the Silver SequenceTM DNA Sequencing kit (Promega) with the FEMS Microbiol Lett 349 (2013) 144–152a 2013 Federation of European Microbiological Societies. ..

    DNA Sequencing:

    Article Title: Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted repeats in Streptomyces plasmid pSV1.
    Article Snippet: .. The sequence ladder was made using the Silver SequenceTM DNA Sequencing kit (Promega) with the labelled primer P843. .. Overexpression and purification of RepA protein The pSV1 repA gene (428–913 bp) was cloned into the NdeI and EcoRI sites of E. coli plasmid pET28b (Novagen) to generate pCY40, and then, it was introduced into E. coli strain BL21 (DE3).

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes
    Article Snippet: .. The sequencing ladder is generated from the PCR DNA template by employing the same end-labelled primer used for reverse transcription ( section 6.1.1.2 ) using the Silver sequenceTM DNA sequencing kit (Promega) according to the manufacturer instructions. ..

    Article Title: Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted repeats in Streptomyces plasmid pSV1.
    Article Snippet: .. The sequence ladder was made using the Silver SequenceTM DNA Sequencing kit (Promega) with the FEMS Microbiol Lett 349 (2013) 144–152a 2013 Federation of European Microbiological Societies. ..

    Generated:

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes
    Article Snippet: .. The sequencing ladder is generated from the PCR DNA template by employing the same end-labelled primer used for reverse transcription ( section 6.1.1.2 ) using the Silver sequenceTM DNA sequencing kit (Promega) according to the manufacturer instructions. ..

    Polymerase Chain Reaction:

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes
    Article Snippet: .. The sequencing ladder is generated from the PCR DNA template by employing the same end-labelled primer used for reverse transcription ( section 6.1.1.2 ) using the Silver sequenceTM DNA sequencing kit (Promega) according to the manufacturer instructions. ..

    Reverse Transcription:

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes
    Article Snippet: .. The sequencing ladder is generated from the PCR DNA template by employing the same end-labelled primer used for reverse transcription ( section 6.1.1.2 ) using the Silver sequenceTM DNA sequencing kit (Promega) according to the manufacturer instructions. ..



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    stop solution from the silver sequencetm dna sequencing kit  (Promega)
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    Promega stop solution from the silver sequencetm dna sequencing kit
    Stop Solution From The Silver Sequencetm Dna Sequencing Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Analysis of 5' ends of leuX tRNA primary transcripts by primer extension [24]. All E. coli strains used in this study were derived from MG1693 (rph-1), which was considered wild-type. MG1693 has a single base pair deletion in the rph gene resulting in a frame-shift mutation [31]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide <t>sequencing</t> gel. A leuX <t>DNA</t> sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.
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    Analysis of 5' ends of leuX tRNA primary transcripts by primer extension [24]. All E. coli strains used in this study were derived from MG1693 (rph-1), which was considered wild-type. MG1693 has a single base pair deletion in the rph gene resulting in a frame-shift mutation [31]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide <t>sequencing</t> gel. A leuX <t>DNA</t> sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.
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    silver sequencetm dna sequencing systems kit  (Promega)
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    Analysis of 5' ends of leuX tRNA primary transcripts by primer extension [24]. All E. coli strains used in this study were derived from MG1693 (rph-1), which was considered wild-type. MG1693 has a single base pair deletion in the rph gene resulting in a frame-shift mutation [31]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide <t>sequencing</t> gel. A leuX <t>DNA</t> sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.
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    Image Search Results


    Analysis of 5' ends of leuX tRNA primary transcripts by primer extension [24]. All E. coli strains used in this study were derived from MG1693 (rph-1), which was considered wild-type. MG1693 has a single base pair deletion in the rph gene resulting in a frame-shift mutation [31]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.

    Journal: Methods (San Diego, Calif.)

    Article Title: Analysis of post-transcriptional RNA metabolism in prokaryotes

    doi: 10.1016/j.ymeth.2018.11.006

    Figure Lengend Snippet: Analysis of 5' ends of leuX tRNA primary transcripts by primer extension [24]. All E. coli strains used in this study were derived from MG1693 (rph-1), which was considered wild-type. MG1693 has a single base pair deletion in the rph gene resulting in a frame-shift mutation [31]. The genotypes of the strains are noted at the top of the lane. Total RNA isolated from all the strains were reverse transcribed using a 32P-end labelled leuX tRNA specific primer and the cDNAs were separated on a 6% polyacrylamide sequencing gel. A leuX DNA sequencing ladder (CTAG) generated by same leuX tRNA specific primer was also separated along with the cDNAs and was used to identify the transcription initiation site (I) and the mature 5' end (II). The primer extension analysis also identified several RNase E cleavage sites (indicated as *) in the rnpA49 mutant, which disappeared in the rne-1 rnpA49 strain.

    Article Snippet: The sequencing ladder is generated from the PCR DNA template by employing the same end-labelled primer used for reverse transcription ( section 6.1.1.2 ) using the Silver sequenceTM DNA sequencing kit (Promega) according to the manufacturer instructions.

    Techniques: Derivative Assay, Mutagenesis, Isolation, Reverse Transcription, Sequencing, DNA Sequencing, Generated